Appearance (Colour)
Yellow with greenish tinge
Appearance (Form)
Free flowing, homogeneous powder
Solubility
80.41 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
8.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear solution forms in tubes which acquires greenish tinge at the surface on standing
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 45°C for 18 - 24 hours
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good, Acid production : + (yellow)
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
For the identification of enterococci from water by the confirmatory test.
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
5.00
Yeast extract
5.00
Dextrose
5.00
Sodium azide
0.40
Methylene Blue
0.01
Sodium chloride
65.00
Directions
1. Add 80.41 gm powder to 1.0 litre distilled/purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
