Appearance (Colour)
Light tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility (Colour) 1% aq. solution
(2%) Light to medium amber, clear
pH (25°C)
6.5 ± 0.5 (2.0%)
Stability (After autoclaving)
(2%) Light to medium amber, clear
Loss on drying
< 5.0 %
NaCl
< 5.0%
Ash
< 12.0%
Nitrogen content (N)
12.0 - 13.0%
Total Aerobic microbial count
(By Plate Count Method) : <= 10, 000 cfu/gm
Total Yeast and Mould count
(By Plate Count Method) : <= 100 cfu/gm
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Escherichia coli ATCC 25922
Result : Positive
Applications
Used for cultivation of highly fastidious microorganisms like Streptococci, Pneumococci, Meningococci, Haemophilus, Neisseria sp. and pathogenic fungi. A highly nutritious mixture of calf brain infusion and beef heart infusion.