Appearance (Colour)
Light to medium amber
Appearance (Form)
Free flowing, homogeneous powder
Solubility
49.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5 % agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber, turns slightly red due to aeration on standing
Cultural Response
Inoculate and Incubate at 35°-37°C for 40-48 hours under anaerobic conditions
Organism
Clostridium botulinum ATCC 25763
Inoculum (cfu) 10-100
Growth : Good, Recovery : >=50%
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth : Good, Recovery : >=50%
Organism
Clostridium sporogens ATCC 11437
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate: >=50%
Applications
Used for the growth and cultivation of anaerobic microorganisms.
Composition
Ingredients
gm/lt.
Enzymatic digest of casein
15.00
L-Cystine
0.50
Dextrose
5.50
Yeast extract
5.00
Sodium chloride
2.50
Sodium thioglycollate
0.50
Resazurin
0.001
Agar
20.00
Directions
1. Add 49.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 151°C for 15 minutes.
