Appearance (Colour)
Pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
55.09 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Greenish brown
Cultural Response
Inoculate and incubate at 37°C for 24 - 48 hours
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%, Colony colour : Pinkish white
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Poor- Good, Recovery Rate : 30-40%,Colony colour : Reddish-pink
Organism
Escherichia coli ATCC 25922
Inoculum
>=103,Growth : Poor, Recovery Rate : 0-10%, Colony colour : Yellow - green
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=103,Growth : Inhibited
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%,Colony colour : Pinkish white
Applications
For the selective isolation of Salmonella other than S.typhi from faeces, food and dairy products.
Composition
Ingredients
gm/lt.
Peptone
5.00
Agar
20.00
Lactose
10.00
Sodium chloride
5.00
Tryptone
5.00
Sucrose
10.00
Brilliant Green
0.0125
Phenol Red
0.08
Directions
1. Add 55.09 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Avoid overheating.
