Appearance (Colour)
Light beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
37.05 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at the following temperature for 24 hours.
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth/Gas production at 35°C : Good/ +ve
Growth/Gas production at 44.5°C : Good/ +ve
Fluorescenc : +ve
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth/Gas production at 35°C : Good/ +ve
Growth/Gas production at 44.5°C : Inhibited/-ve
Fluorescenc : -ve
Organism
Enterococcus faecalis ATCC 19433
Inoculum (cfu) 10-100
Growth/Gas production at 35°C : Inhibited/-ve
Growth/Gas production at 44.5°C : Inhibited/-ve
Fluorescenc : -ve
Applications
Used for the fluorogenic detection of Escherichia coli in water, food and milk samples.
Composition
Ingredients
gms/lt.
Tryptose
20.00
Lactose
5.00
Sodium chloride
5.00
Bile salts mixture
1.50
Dipotassium phosphate
4.00
Monopotassium phosphate
1.50
4-Methylumbelliferyl-b-D-glucuronide
0.05
Directions
1. Add 37.05 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into tubes containing inverted Durham`s tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
Notes
EC Medium w/MUG is not intended for use in the diagnosis of disease or other conditions in humans.
