Appearance (Colour)
White, fine powder
Solubility
Soluble in 0.2 N sodium hydroxide
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hours. (Aseptically add the rehydrated contents of 1 vial of Nalidixic Selective Supplement as per direction)
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation of Pseudomonas aeruginosa.
Composition
Nalidixic acid
15.00 mg/vial
Directions
1. Aseptically add 5ml of sterile 0.2 N Sodium Hydroxide to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and pour in petriplates.
3. Add 5ml (1 vial) of supplement to 1000 ml of media- Cetrimide Agar, Base(45470).
Media used - Cetrimide Agar Base.
