Appearance (Colour)
Brownish yellow
Solubility (Colour) 2% aq. solution
Completely soluble in distilled water
pH (25°C)
7.0 ± 0.5 (2.0%)
Stability (After autoclaving)
Medium amber to yellowish brown, clear
Loss on drying
< 5.0 %
Nitrogen content (N)
9.0 - 11.0 %
NaCl
< 5.0%
Ash
11.0 - 16.0%
Total Aerobic microbial count
(By Plate Count Method) : <= 10,000 cfu/gm
Total Yeast and Mould count
(By Plate Count Method) : <= 100 cfu/gm
Cultural Response
Growth Promotion Parameters : Inoculate and incubate at 37°C for 18-24 hours with the following organisms
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Tryptophan content
Escherichia coli ATCC 25922 (PassesTest : Positive)
ATCC 25922
Result : Positive
Applications
Water soluble portion of autolysed yeast especially rich in B-complex vitamins, used in microbiological and fermentation media.
