Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Activity
Enzyme is tested in ligation of Lambda DNa digests at 16°C within one hour.
Description
T4 DNA ligase is an ATP-dependent recombinant enzyme, isolated from Escherichia coli, used for DNA cloning. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. It will join both blunt-ended and cohesive-ended restriction fragments of DNA as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Applications
Joins DNA molecules with compatible cohesive termini.
Joins blunt-ended double stranded DNA molecules to one another or to synthetic linkers.
Gene cloning - recombination of vector and insert.
Includes
250 U (Weiss):
T4 DNA Ligase RC - 50 μl
10X Ligation Buffer - 1.5 ml
1000 U(Weiss):
T4 DNA Ligase RC - 200 μl
10X Ligation Buffer - 2 X 1.5 ml
5000 U(Weiss):
T4 DNA Ligase RC - 1000 μl
10X Ligation Buffer - 5 X 1.5 ml
