Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
39.50 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.2% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
blood agar base no.2Medium to dark amber
(Note : After addition of 5% sterile defibrinated blood: Cherry red, opaque)
Cultural Response
Inoculate and incubate at 37°C for 18-48 hours.
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth wo/blood : Good
Recovery Rate : >=70%
Growth w/blood : Good
Recovery Rate : >=70%
Haemolysis : Beta
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth wo/blood : Fair-Good
Recovery Rate : ~50%
Growth w/blood : Good
Recovery Rate : >=70%
Haemolysis : Beta
Organism
Streptococcus pneumoniae ATCC 6303
Inoculum (cfu) 10-100
Growth wo/blood : Fair- Good
Recovery Rate : ~50%
Growth w/blood : Good
Recovery Rate : >=70%
Haemolysis : Alpha
Organism
Neisseria meningitidis ATCC 13090
Inoculum (cfu) 10-100
Growth wo/blood : Fair- Good
Recovery Rate : ~50%
Growth w/blood : Good
Recovery Rate : >=70%
Haemolysis : None
Applications
For the isolation, cultivation and detection of haemolytic activity of streptococci, pneumococci and other particularly fastidious microorganisms.
Composition
Ingredients
gms/lt.
Agar
12.00
Sodium chloride
5.00
Proteose peptone
15.00
Yeast extract
5.00
Liver extract
2.50
Directions
1. Add 39.50 gm powder to 1.0 liter distilled/purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 5-10% sterile defibrinated blood and mix thoroughly.
6. Dispense into sterile petri plates.
