Appearance (Colour)
Light beige, may have a slight green cast
Appearance (Form)
Free flowing, homogeneous powder
Solubility
75.66 gm/liter.
Solubility before autoclaving (Clarity)
Slightly opalescent.
Gel strength
Firm, comparable with 1.5 % Agar gel.
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent.
Prepared Medium Appearance after autoclaving (Colour)
Green with yellowish cast.
Cultural Response
Incubate at 35 ± 2°C for 18-24 hours.
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : ~50.0%
Colony colour : greenish blue with black centres.
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Fair - Good
Recovery Rate :30.0%
Colony colour : salmon orange,may have bile precipitate.
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Fair - Good
Recovery Rate :30.0%
Colony colour : salmon orange.
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :~40.0 - 50.0%
Colony colour : greenish blue
Applications
Used for the isolation and cultivation of gram negative enteric microorganisms from a variety of clinical and non clinical specimens.
Composition
Ingredients
gms/lt.
Proteose peptone
12.00
Yeast extract
3.00
Bile salts no. 3
9.00
Lactose
12.00
Sucrose
12.00
Salicin
2.00
Sodium chloride
5.00
Sodium thiosulfate
5.00
Ferric ammonium citrate
1.50
Agar
15.00
Bromothymol Blue
0.065
Acid Fuchsin
0.10
Directions
1. Add 75.66 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Do not autoclave.
5. Dispense into sterile petri plates.
