Appearance (Colour)
Cream to greenish yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
89.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
8.6 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Green
Cultural Response
Inoculate and incubate at 35 - 37°C for 18- 24 hours
Organism
Vibrio cholera ATCC 9459
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Yellow
Organism
Vibrio parahemolyticus ATCC 17802
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Green
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from clinical specimens.
Composition
Ingredients
gm/lt.
Sucrose
20.00
Agar
15.00
Veg. Peptone
10.00
Sodium citrate
10.00
Sodium thiosulphate
10.00
Sodium chloride
10.00
Synthetic detergent
8.00
Yeast extract
5.00
Ferric citrate
1.00
Bromothymol Blue
0.04
Thymol Blue
0.04
Directions
1. Add 89.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave or overheat.
