Appearance (Colour)
Yellow
Appearance (Form)
Crystalline powder
Activity
min. 6000 units/gm powder
Unit Definition
One unit will oxidatively deaminate 1.0 micromole of D-alanine to pyruvate per minute at pH 8.3 at 25°C, in the presence of catalase. (This is equivalent to an O2 uptake of approximately 335 µL in 30 minutes.)
Enzyme solution for activity determination
Prepared in 3.6 M ammonium sulphate solution, pH 6.5.