Appearance (Colour)
Cream
Appearance (Form)
Free ,flowing homogeneous powder
Solubility (Colour) 2% aq. solution
Light to medium amber, clear
pH (25°C)
5.50 - 6.50 (2%)
Stability (After autoclaving)
Light to medium amber, clear
Loss on drying
< 5.0 %
Nitrogen content (N)
9.0 % - 11.0 %
NaCl
< 15.0 %
Ash
< 30.0 %
Total Aerobic microbial count
(By Plate Count Method) : <10,000 cfu/gm
Total Yeast and Mould count
(By Plate Count Method) : <100 cfu/gm
Cultural Response
Inoculate and incubate at 37° C for 18 - 48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Escherichia coli ATCC 25922
Growth : Good, Result : Negative
Applications
Acid digest of casein, a rich source of nitrogen recommended for use in microbiological culture media that require a completely hydrolyzed protein as a nitrogen source.
