Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
25.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.6 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 48 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Applications
Recommended nutritive medium for mass cultivation of bacteria for vaccine production.
Composition
Ingredients
gm/lt.
Peptone
10.00
Sodium chloride
5.00
Beef extract (ex. buffalo)
10.00
Directions
1. Add 25.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
