Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
20.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37 ± 2°C for 18 - 24 hrs
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Bacillus cereus ATCC 10876
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Lactococcus lactis species ATCC 19435
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Listeria monocytogenes ATCC 19118
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Applications
For the enumeration of bacteria in milk and dairy products.
Composition
Ingredients
gm/lt.
Tryptone
5.0
Yeast extract
2.5
Skim milk
1.0
Dextrose
1.0
Agar
10.5
Directions
1. Add 20.00 gm powder to 1000 ml distilled/purified water and mix thoroughly.
2. Allow to stand for 15 min.
3. Gently heat and bring to boiling with frequent agitation.
4. Autoclave at 15 psi pressure at 121°C for 15 min.
