Appearance (Colour)
Off white to cream
Appearance (Form)
Free flowing homogeneous powder
Solubility
1.637 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent, may have presence of slight precipitate
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent, may have presence of slight precipitate
Prepared Medium Appearance after autoclaving (Colour)
Colourless
Cultural Response
Inoculate and incubate at 20-25°C for 1 week
Organism
Synechcystis species PCC6803 ATCC 27184
Inoculum (cfu) 10-100
Growth : Good
Applications
For culture and maintenance of cyanobacteria.
Composition
Ingredients
gm/lt.
Sodium nitrate
1.50
Dipotassium hydrogen phosphate
0.0314
Magnesium sulphate
0.036
Calcium chloride
0.0367
Sodium carbonate
0.020
Disodium magnesium EDTA
0.001
Citric acid
0.0056
Ferric ammonium citrate
0.006
Directions
1. Add 1.637 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15psi pressure at 121°C for 15 minutes.
Note : For marine species make a solution of 10 g/L sodium chloride and 1 µg/L Vitamin B12 Add 20 ml of this solution (sterile-filtered) to 1 litre medium.
