Appearance (Colour)
Pinkish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
51.53 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish purple
Cultural Response
Inoculate and incubate at 35°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Colony colour : pink w/bile precipitate
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : pink to red
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Colony colour : colourless
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Fair-good
Recovery rate : > 40.0%
Colony colour : pale pink to red
Organism
Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Colony colour : colourless
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Colony colour : colourless
Organism
Salmonella paratyphi A
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Colony colour : colourless
Organism
Salmonella paratyphi B
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Colony colour : colourless
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=103,Growth : Inhibited
Applications
For the selective isolation, cultivation and differentiation between coliforms and non -lactose fermentors.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
20.00
Bile salts no. 3
1.50
Lactose
10.00
Sodium chloride
5.00
Neutral Red
0.03
Crystal Violet
0.001
Agar
15.00
Directions
1. Add 51.53 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
