Appearance (Colour)
Light cream to yellow coloured
Appearance (Form)
Free flowing, homogeneous powder
Solubility
72.00 gm/liter (for double strength media)
Solubility before autoclaving (Clarity)
Clear to opalescent
Gel strength
Firm, comparble with 1.5% Agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent. After addition of Haemoglobin: Chocolate brown coloured opaque
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber.
Cultural Response
Inoculate and incubate at 35-37°C for 40-48 hours at 5-10.0 % carbondioxide and 70.0% humidity
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Organism
Streptococcus peumoniae ATCC 6303
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Organism
Haemophilus influenzae ATCC 19418
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Organism
Neisseria gonorrhoeae ATCC 19424
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Organism
Neisseria meningitidis ATCC 13090
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >= 50.0%
Applications
Used for isolation and cultivation of Gonococci.
Composition
Ingredients
gms/lt.
Peptone, special 15.00
15.00
Corn starch 1.00
1.00
Dipotassium phosphate 4.00
4.00
Monopotassium phosphate 1.00
1.00
Sodium chloride 5.00
5.00
Agar 10.00
10.00
Directions
1. Add 7.20 gm powder to 100 ml of distilled/purified water and mix thoroughly for double strength media.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4 .Cool to 45-500C.Aseptically add prepared Haemoglobin solution (100 ml of 2.0% solution and GC supplement with antibiotics). Mix well.
