Appearance (Colour)
Pinkish tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
36.54 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
6.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Pink red
Cultural Response
Inoculate and incubate at 25-30°C for 24- 48 hours(or upto 4 days)
Organism
Aspergillus niger ATCC 16404
Inoculum
Growth (Plain) : Good
Growth with Chlortetracycline : Good
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth (Plain) : Good
Recovery Rate : >=50.0%
Growth with Chlortetracycline : Good
Recovery Rate : >=50.0%
Organism
Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100
Growth (Plain) : Good
Recovery Rate : >=50.0%
Growth with Chlortetracycline : Good
Recovery Rate : >=50.0%
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth (Plain) : Good
Growth with Chlortetracycline : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
>=103,Growth (Plain) : Inhibited
Growth with Chlortetracycline : Inhibited
Applications
Used for the selective cultivation and isolation of fungi.
Composition
Ingredients
gm/lt.
Soya peptone
5.00
Dextrose
10.00
Monopotassium phosphate
1.00
Magnesium sulfate
0.50
Agar
20.00
Rose Bengal
0.035
Directions
1. Add 36.54 gm powder to 1000 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling .
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45°C. If desired add 35µg of Chlortetracycline per ml of medium & mix well.
