Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
28.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Incubate at 37°C ± 2°C for 18-24 hours
Organism
Escherchia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0%
Organism
Staphylococcus aureus
ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0%
Organism
Pseudomonas aeruginosa
ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0%
Organism
Streptococcus pyogenes
ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0%
Organism
Salmonella typhimurium
ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0%
Applications
General purpose (animal origin free) medium for the cultivation of micro organisms.
Composition
Ingredients
gm/lt.
Veg. peptone
5.00
Veg. extract
1.50
Yeast extract
1.50
Sodium chloride
5.00
Agar
15.00
Directions
1. Add 28.00gm powder to distilled/purified water.
2. Bring volume to 1 liter and mix thoroughly.
3. Gently heat ad bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 45-50°C.
6. If desired, aspetically add sterile 5.0 -10.0% defibrinated blood.
