Appearance (Clarity)
Clear
Appearance (Colour)
Yellow to brown
Appearance (Form)
Liquid
Composition
1.5% solution of 4-nitro blue tetrazolium chloride (NBT) in 70% N,N-dimethylformamide (DMF)
Suitability as chromogenic substrate for Alkaline phosphatase
Passes test
Application
Applications:
It is a chromogenic substrate for enzyme alkaline phosphatase, producing a blue coloured precipitate at the site of enzymatic activity. It has applications in immunohistochemistry and immunocytochemistry.