Appearance (Colour)
White to off-white
Appearance (Form)
Crystalline powder
Solubility (Turbidity) 1% aq. solution
Clear to slight opalascent
Activity
min. 1000 BAEE units/mg powder
Suitability for Cell Culture
Passes
Bacterial Endotoxin Test (BET) (EU/mg = IU/mg)
<0.05 EU/mg
Unit Definition
One BAEE unit of Trypsin activity will produce a ΔA at 253 nm of 0.001 per minute with BAEE as substrate at pH 8.0 at 25 °C in a reaction volume of 3.0 mL.
3 BAEE units = 1 NFunit
Enzyme solution for activity determination
Prepared in 0.001 N Tris HCl.
