Appearance (Colour)
Tan
Appearance (Form)
Free flowing,homogeneous powder.
pH (25°C)
(5.1 % aq. Solution)
5.90 - 6.30
Solubility
(Before autoclaving|)
Medium to dark amber,very slightly opalescent w/o precipitate.
Gel strength
Firm, comparable with 1.1% gel strength.
Prepared Medium Appearance after autoclaving (Colour)
Medium to dark amber
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent w/o precipitate
Cultural Response
Inoculate and incubate at 37°C for 40 - 48 hours.
Organism
Lactobacillus acidophilus ATCC 4356
Inoculum (cfu) 10-100
Growth : Good
Recovery : >= 70.0%
Organism
Lactobacillus casei ATCC 9595
Inoculum (cfu) 10-100
Growth : Good
Recovery : >= 70.0%
Organism
Staphylococus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery : >= 70.0%
Applications
Used for the cultivation of lactobacilli.
Composition
Ingredients
gm/lt.
Tomato juice (400 ml)
20.00
Peptone
10.00
Peptonized milk
10.00
Agar
11.00
Directions
1. Add 51.00 gm powder to 1000 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
