Appearance (Colour)
Cream to greenish yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.97 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Green.
After addition of 5.0% egg yolk emulsion: Yellowish green, opaque
Cultural Response
Inoculate and incubate at 37°C for 24 hours with the following
Organism
Bacillus cereus ATCC 10876
Inoculum (cfu) 10-100
Growth : Good, Recovery rate : >=50.0%,
Colony colour :Blue,
Egg Yolk Reaction : Precipitation
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good, Recovery rate : >=50.0%,
Colony colour : Yellow,
Egg Yolk Reaction : Clearing
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth :Good, Recovery rate : >=50.0%,
Colony colour :Green,
Egg Yolk Reaction : -
Organism
Escherichia coli ATCC 25922
Inoculum
>=103,Growth : Inhibited
Applications
Used for the isolation and enumeration of Bacillus cereus.
Composition
Ingredients
gm/lt.
Peptone
1.00
Mannitol
10.00
Sodium chloride
2.00
Magnesium sulphate
0.10
Disodium phosphate
2.50
Monopotassium phosphate
0.25
Sodium pyruvate
10.00
Bromothymol Blue
0.12
Agar
15.00
Directions
1. Add 20.50 gm powder to 475.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 50°C. Aseptically add 25 ml of sterile egg yolk emulsion and rehydrated contents of 1 vial of Polymixin B Selective Supplement. (35980).
