Dehydrated Appearance
Light yellow to pink,free flowing homogeneous powder
Solubility before autoclaving (Clarity)
Red, very slightly opalescent
Prepared Medium Appearance
Red, very slightly opalescent
pH (25°C)
7.4 ± 0.2
Cultural Response
Inoculate and incubate at 37 °C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Colour of colony : Green
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Colour of colony : Yellow, mucoid
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>103, Growth : Inhibited
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth:Good
Colour of colony : Pink
Applications
For the differentiation and enumeration of Escherichia coli and other coliforms by membrane filter technique.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
40.00
Yeast extract
6.00
Lactose
30.00
Phenol Red
0.20
Chromogen
0.20
Sodium lauryl sulphate
1.00
Sodium pyruvate
0.50
Agar
10.00
Directions
1. Add 87.90 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
