Appearance (Clarity)
Clear
Appearance (Form)
Colourless to pale brown liquid
Composition
1% solution of 5-bromo-4-chloro-3-indolylphosphate-p-Toluidine salt (BCIP) in N,N-dimethylformamide (DMF)
Suitability as chromogenic substrate
Passes
Application
Applications:
It is a chromogenic substrate for enzyme alkaline phosphatase, producing a blue coloured precipitate at the site of enzymatic activity. It has applications in immunohistochemistry and immunocytochemistry.