Appearance (Colour)
Dark green
Appearance (Form)
Crystalline powder
Assay (UV)
min.50%
Loss on drying
max. 10%
Absorbance (A) of 1% solution in 50% Ethanol in a 1cm cell
.
@550nm
min.1580
Application
Applications:
- Pyronin Y solutions are red in transmitted light, displaying a yellow fluorescence in reflected light.
- Used widely in combination with methyl green to selectively and differentially stain nucleic acids. (In the dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains the DNA green.)
- Used in correlating RNA content by flow cytometry.
