Appearance (Colour)
Biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
55.50 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent gel with a bluish tinge
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent gel with a bluish tinge
Prepared Medium Appearance after autoclaving (Colour)
Dark amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hours
Organism
Listeria monocytogenes ATCC 19112
Inoculum (cfu) 10-100
Growth : Good,
Recovery rate : >=50%,
Esculin hydrolysis : Positive reaction, blackening of medium around colony
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good,
Recovery rate :>=40.0 - 50.0%,
Esculin hydrolysis : Negative reaction
Organism
Escherichia coli ATCC 25922
Inoculum
>=103,Growth : Inhibited
Organism
Bacillus subtilis ATCC 6633
Inoculum
>=103,Growth : Inhibited
Applications
Used for isolation of Listeria species from pathological samples.
Composition
Ingredients
gm/lt.
Neopeptone
23.00
Sodium chloride
5.00
Corn Starch
1.00
Esculin
1.00
Lithium chloride
15.00
Ammonium Ferric citrate
0.50
Agar
10.00
Directions
1. Add 27.75 gm powder to 500 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 121 °C at 15 psi pressure for 15 minutes.
4. Aseptically,add the rehydrated contents of 1 vial of Oxford Listeria Supplement (42116) or 1 vial of Listeria Moxalactam Supplement (15650).
5. Mix well and dispense.
