Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Absence of nucleases
Tested using relevant procedures
Amplification capability
Tested by RT-PCR
Description
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA
strand.
This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.
The enzyme has increased thermal stability, that allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ to 5’ exonuclease and reduced RNaseH activity, that improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNa synthesis up to 10kb long.
Applications
PCR,
real-time PCR
second strand cDNA synthesis
DNA labeling
Analysis of RNA by primer extension
Includes
5000U
· M-MLV (Recombinant) (200U/μl ) - 25 μl
· Reaction Buffer (10X) - 50 μl
10000U
· M-MLV (Recombinant) (200U/μl ) - 50 μl
· Reaction Buffer (10X) - 100 μl
