Appearance (Colour)
Pink
Appearance (Form)
Coarse, free flowing, homogeneous powder
Solubility
155.0 gm/liter
Solubility before autoclaving (Colour)
Red
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Colour)
Red
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Cultural Response
Inoculate and incubate at 37°C for 24- 48 hours.
For Gelatin liquefaction results....(after incubation)refrigerate tubes at ~5°C for 2 hours, and compare to non-inoculated tubes.
Organism
Clostridium perfringens
ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Lactose fermentation : (+)ve
Gelatin liquefication : (+)Ve
Organism
Clostridium sporogenes
ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Lactose fermentation :(-)ve
Gelatin liquefication : (+) ve
Key
Lactose fermentation : (+)ve : colour of medium changes to yellow
Lactose fermentation : (-)ve : no change in colour of medium
Gelatin liquefaction : (+)ve : medium turns to liquid
Gelatin liquefaction : (-)ve : medium remains solidified
Applications
For the cultivation of Clostridium perfringens.
Composition
Ingredients
gms/lt.
Gelatin
120.00
Tryptose
15.00
Lactose
10.00
Yeast extract
10.00
Phenol Red
0.05
Directions
1. Add 155.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
