Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
29.5 gm/lit
Solubility before autoclaving (Clarity)
Light amber, clear to very slightly opalescent
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent, solution with upper 10% or less medium having pinkish tinge on standing
Prepared Medium Appearance after autoclaving (Colour)
Light amber coloured
Cultural Response
Inoculate and incubate at 37° C ± 2°C for 18-48 hours
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth- good
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth- good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth- good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth- good
Organism
Corynebacterium diphtheriae ATCC 11913
Inoculum (cfu) 10-100
Growth- good
Applications
Used for the cultivation of aerobic and anaerobic bacteria. It is also used for sterility testing.
Composition
Ingredients
gm/lt.
Casein enzymatic hydrolysate
15.00
Yeast extract
5.00
Sodium hydrosulphite
0.5
Sodium chloride
2.5
Dextrose
5.5
Resazurin
0.001
Agar
1.00
Directions
1. Add 29.5 gm powder to distilled/purified water.
2. Bring volume to 1 liter & mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
