Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
51.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.35% agar gel
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light yellow
Cultural Response
Inoculate and incubate at 35 ± 2°C for 40-48 hours, under anaerobic conditions
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Bacteroides fragilis ATCC 23745
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Clostridium butyricum ATCC 9690
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Applications
Used for the cultivation and enumeration of Clostridium species and other anaerobes from clinical specimens and foods.
Composition
Ingredients
gms/lt.
Tryptone
10.00
Beef extract (ex.buffalo)
10.00
Sodium acetate
3.00
Yeast extract
3.00
Soluble starch
1.00
Dextrose
5.00
L-Cysteine hydrochloride
0.50
Sodium chloride
5.00
Agar
13.50
Directions
1. Add 51.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 10 psi pressure at 115°C for 15 minutes.
4. Dispense in sterile petri plates.
