Appearance (Colour)
Off white to light tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
39.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours. Streak on Mac Conkey agar plates from GN Broth tubes. Incubate plates at 35 ± 2°C for 18-24 hours.
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth in GN Broth : Good
Growth on MacConkey agar : Good
Colony colour : colourless
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth in GN Broth : Good
Growth on MacConkey agar : Good
Colony colour : pink to red w/bile precipitate
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth in GN Broth : Good
Growth on MacConkey agar : Good
Colony colour : colourless
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth in GN Broth : Good
Growth on MacConkey agar : Good
Colony colour : colourless
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth in GN Broth : Good
Growth on MacConkey agar : Good
Colony colour : colourless
Organism
Enterococcus faecalis ATCC 19433
Inoculum (cfu) 10-100
Growth in GN Broth : none -poor
Growth on MacConkey agar : none -poor
Colony colour : pale pink-red
Applications
For the selective cultivation of gram negative microorganisms.
Composition
Ingredients
gms/lt.
Pancreatic digest of casein
12.00
Proteose peptone no. 3
8.00
Dextrose
1.00
D-Mannitol
2.00
Sodium citrate
5.00
Sodium deoxycholate
0.50
Dipotassium phosphate
4.00
Monopotassium phosphate
1.50
Sodium chloride
5.00
Directions
1. Add 39.00 gm powder to 1.0 liter of distilled/puriifed water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes or flasks as desired.
4. Autoclave at 10 lbs pressure at 115°C for 15 minutes.
5. Avoid Overheating.
