Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Assay
Plasmid DNA digested with Restriction enzyme is treated with Ligase enzyme to check if dephophorylation prevented Ligation.
Absence of nucleases
Incubated with super-coiled Plasmid DNA, ds DNA ss DNA and RNA samples at 37°C
Description
Shrimp Alkaline Phosphatase (SAP) is a multi-purpose alkaline phosphatase that can be fully inactivated by a short heat pulse. This simplifies most protocols for dephosphorylation.The native form of SAP has been the gold standard for dephosphorylation, and is now replaced by a recombinant version of the same enzyme, rSAP. rSAP is free of any contaminating enzymatic activity. Produced in Pichia pastoris strain expressing a recombinant Alkaline phosphatase gene of shrimp (Pandalus borealis).
Includes
500 Units
Shrimp Alkaline Phosphatase (rSAP) (1u/μ l) – 0.5ml
1000 Units
Shrimp Alkaline Phosphatase (rSAP) (1u/μ l) – 1.0ml
