Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
45.26 gram/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Colour)
Dark purple w/red cast
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Cultural Response
Inoculate and Incubate at 37°C ± 2°C for 2 hours and at 44-45°C for 18 - 22 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum
Growth : Inhibited
Applications
Recommended medium for isolation, differentiation and enumeration of Escherichia coli from water by membrane filtration.
Composition
Ingredients
gm/lt.
Proteose peptone
5.00
Yeast extract
3.00
Lactose
10.00
Sodium chloride
7.50
Sodium lauryl sulphate
0.20
Sodium deoxycholate
0.10
Potassium dihydrogen phosphate
1.00
Dipotassium hydrogen phosphate
3.30
Bromocresol Purple
0.080
Bromophenol Red
0.080
Agar
15.00
Directions
1. Add 45.26 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
