Appearance (Colour)
Clear Solution
Solubility
40%, Clear solution
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours. (Aseptically add the contents of vial as per direction.) Growth on Urea Agar Base (88708)
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Urease production : Positive
Organism
Klebsiella pneumoniae ATCC13883
Inoculum (cfu) 10-100
Growth : Good
Urease production : Positive
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Urease production : Negative
Applications
Used for the detection of urease activity.
Composition
Urea
4.00 gm
Distilled water
10.00 ml
Directions
1. Aseptically add to sterile cooled media at 40-45°C.
2. Mix well and dispense as desired.
3. Add 50 ml of urea solution in 950 ml media:
Urea Agar Base (88708, 42465,18339),
Urea Broth Base (97438),
Hemmes Medium Base (20852),
MIU Medium Base (16309),
Yersinia Identification Broth Base.
4. Add 25 ml of urea solution in 975 ml of media : Kohn Two Tube Medium No.1 Base.
