Appearance (Colour)
Light biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
46.40 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=50%,
Pigment Production : Blue-green colonies, greenish pigment
diffusing into the surrounding medium;
fluorescence under UV light
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%,
Pigment Production : Blue-green colonies, greenish pigment diffusing into the surrounding medium;
fluorescence under UV light
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Fair -Good, Recovery Rate : >=40 - 50%, Pigment Production : No pigmentation observed
Applications
For the isolation, cultivation and differentiation of Pseudomonas species on the basis of pyocyanin production.
Composition
Ingredients
gm/lt.
Proteose peptone No.3
20.00
Magnesium chloride
1.40
Potassium sulphate
10.00
Agar
15.00
Directions
1. Add 10 gm of glycerol to distilled/purified water.
2. Add 46.40 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
