Appearance (Colour)
Grey to black
Appearance (Form)
Free flowing, homogeneous powder
Solubility
45.5 gm/liter
Gel strength
Firm, comparable with 1.2% agar gel
Prepared Medium Appearance before autoclaving (Clarity)
Opaque gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opaque gel
Prepared Medium Appearance after autoclaving (Colour)
Black
Cultural Response
Inoculate and incubate at 42°C for 24-48 hours under microaerophilic conditions
Organism
Campylobacter coli ATCC 33559
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50%
Colony colour : Creamy grey
Organism
Campylobacter jejuni ATCC 29428
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50%
Colony colour : Grey
Organism
Escherichia coli ATCC 25922
Inoculum
>=10³
Growth : Inhibited
Applications
Used for the selective isolation and differentiation of Campylobacter species from food and animal feeding stuffs. This medium is recommended by ISO 10272-1 & 2:2017.
Composition
Ingredients
gms/lt.
Tryptone
3.00
Peptone
10.00
Beef extract (ex. buffalo)
10.00
Sodium chloride
5.00
Sodium deoxycholate
1.00
Sodium pyruvate
0.25
Activated charcoal
4.00
Ferrous sulphate
0.25
Agar
12.00
Directions
1. Add 22.75 gm powder to 500 ml of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4.Cool to 45-50°C, aseptically add rehydrated contents of 1 vial of Campylobacter Supplement.
5. Mix well and pour in sterile petri plates.
