Appearance (Colour)
Beige w/brownish tint
Appearance (Form)
Free, flowing homogeneous powder
Solubility
46.00 gms/lit.
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm,comparable with 2.0% agar gel.
pH (25°C)
7.4 - 7.8
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Cultural Response
Inoculate and incubate at 35 -37°C for 18 - 48 hours with added T.S.C.Supplement with following organisms
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Clostridium perfringens ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Applications
Used for isolation of mesophilic spore forming anaerobes in food spoilage.
Composition
Ingredients
gms/lt.
Tryptose
15.00
Papaic digest of soyabean meal
5.00
Yeast extract
5.00
Ferric ammonium citrate
1.00
Agar
20.00
Directions
1. Add 23.00 gm powder to 500.0 ml distilled/purified water and mix thoroughly.
2. If desired,add 0.5 to 1.0% dextrose.
3. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 10 minutes.
4. Cool to 45-50°C and aseptically,add one vial of T.S.C.Supplement ( 55688 ). Mix well and pour in petriplates.
