Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
24.50 gm/liters
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 30°C for 18-24 hours and also at 6.5°C, if desired
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth(30°C) : Good, Recovery Rate : >=70.0%
Growth(6.5°C) : Good, Recovery Rate : >=70.0%
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth(30°C) : Good, Recovery Rate : >=70.0%
Growth(6.5°C) : Good, Recovery Rate : >=70.0%
Organism
Clostridium prefrigenes ATCC 12924
Inoculum (cfu) 10-100
Growth(30°C) : Good, Recovery Rate : >=70.0%
Growth(6.5°C) : Good, Recovery Rate : >=70.0%
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth(30°C) : Good, Recovery Rate : >=70.0%
Growth(6.5°C) : Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth(30°C) : Good, Recovery Rate : >=70.0%
Growth(6.5°C) : Inhibited
Applications
For cultivation and enumeration of microorganisms encountered in dairy industries.
Composition
Ingredients
gm/lt.
Enzymatic hydrolysate of casein
5.00
Dextrose
1.00
Yeast extract
2.50
Skim milk powder
1.00
Agar
15.00
Directions
1. Add 24.50 gm powder to 1 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
