Appearance (Colour)
White, fine powder
Solubility
Completely soluble in distilled water
Cultural Response
Inoculate and incubate at 37 ± 2°C for 24-48 hours. (Aseptically add rehydrated contents of 1 vial as per direction) Growth on Bacteroides Selective Supplement.
Organism
Bacteroides fragilis ATCC 25285
Inoculum (cfu) 10-100
Growth : Good
Esculin hydrolysis : Positive
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Esculin hydrolysis : Negative
Organism
Proteus mirabilis ATCC 25933
Inoculum
Growth : None-poor
Esculin hydrolysis : Negative
Organism
Clostridium perfringens ATCC 13124
Inoculum
Growth : Inhibited
Esculin hydrolysis : Negative
Applications
Used for the selective isolation of Bacteroides species.
Composition
Gentamicin
50.00mg/vial
Directions
1. Aseptically add 5ml of sterile distilled water to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and pour in sterile petriplates.
3. Add 5ml (1 vial) of supplement in 500 ml of Bacteroides Bile Esculin Agar Base.
