Appearance (Colour)
Purple
Appearance (Form)
Free flowing, homogeneous powder
Solubility
41.50 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent with fine precipitate
Gel strength (1.5% gel)
Firm, comparable with 1.5% gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent with fine precipitate
Prepared Medium Appearance after autoclaving (Colour)
Organish Pink
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Pink to red with metallic sheen
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Pink, mucoid
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Colourless to very light pink
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Pink, mucoid
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Colourless to pale pink
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Colourless to very light pink
Organism
Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Colourless to pale pink
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good, Colony colour : Colourless, irregular
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : None - poor, Colony colour : Pink, small
Applications
For the selective isolation, cultivation and differentiation of coliforms and other enteric microorganisms based on their ability to ferment lactose.
Composition
Ingredients
gm/lt.
Peptone
10.00
Agar
15.00
Lactose
10.00
Sodium sulphite
2.50
Dipotassium hydrogen phosphate
3.50
Basic Fucshin
0.50
Directions
1. Add 41.50 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Dispense into sterile petri plates.
