Appearance (Colour)
White to off - white to pale yellow
Appearance (Form)
Crystalline powder
Assay
min. 97%
Application
2′,7′-Dichlorofluorescein diacetate is a cell-permeable non-fluorescent probe. DCFH is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.