Appearance (Colour)
Light biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
85.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37 ± 2°C for 18-48 hours
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium botulinum ATCC 25763
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for the isolation of Clostridium perfringens from food samples.
Composition
Ingredients
gm/lt.
Yeast extract
20.00
Casein enzymic hydrolysate
50.00
Peptic digest of animal tissue
5.00
Dextrose
4.00
Disodium phosphate
5.00
Sodium thioglycollate
1.00
Directions
1. Add 85.00 gm powder to distilled/purified water.
2 Bring volume to 1.0 liter and mix thoroughly.
3 Gently heat and bring to boiling.
4 Dispense into test tubes/bottles as desired.
5. Tubes : 15 ml into 20 x 150 mm test tubes. Bottles : 100 ml into 170 ml bottles.
6. Autoclave at 15 psi pressure at 121°C for 10-15 minutes. (8-10 minutes for tubes, 15 minutes for bottles).
