Appearance (Colour)
Biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.7 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber. After addition of 5% sterile defibrinated blood: Cherry red, opaque
Cultural Response
Inoculate and incubate at 37°C for 48-72 hours
Organism
Bordetella bronchiseptica ATCC 4617
Inoculum (cfu) 10-100
Growth w/o blood : Good, Growth w/blood : Good, Haemolysis : Gamma
Organism
Bordetella pertussis ATCC 8467
Inoculum (cfu) 10-100
Growth w/o blood : Poor - Fair, Growth w/blood : Good, Haemolysis : Beta
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Applications
For the detection and isolation of Bordetella species from clinical specimens.
Composition
Ingredients
gm/lt.
Agar
20.00
Sodium chloride
5.50
Peptic digest of animal tissue
10.00
Potatoes solids from infusion
125.00
Directions
1. Add 40.00 gm powder to 980 ml distilled/purified water containing 10 ml glycerol.
2. Mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 50°C.
6. Add aseptically 200.0 ml rabbit blood (prewarmed to 35°C) and mix thoroughly.
7. If desired,add 2 vials of Bordfetella Selective Supplement (66006).
