Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Activity
Enzyme is tested in ligation of Lambda DNA digests at 16°C within one hour.
Description
T4 DNA ligase is a polypeptide that catalyzes the formation of phosphodiaster bonds between adjacent 3'-hydroxyl and 5'- phosphate termini in DNA. The enzyme is active on double-stranded DNA, nicked DNA and RNA substrates.
Applications
Joins DNA molecules with compatible cohesive termini.
Joins blunt-ended double stranded DNA molecules to one another or to synthetic linkers.
Gene cloning - recombination of vector and insert
Includes
10000 U (CEU):
T4 DNA Ligase VLC - 500 μl
10X Ligation Buffer - 1.0 ml
40000 U(CEU):
T4 DNA Ligase VLC - 2000 μl
10X Ligation Buffer - 4 X 1.0 ml
