Appearance (Colour)
Cream to Yellowish-grey
Appearance (Form)
Free flowing, homogeneous powder
Solubility
19.31 gm /liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
6.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Purple
Cultural Response
Inoculate and incubate at 35°C -37°C for 18-24 hours.
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Motility : (+)ve
Arginine dihydrolyase : positive reaction,purple clour
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Motility : (+)ve
Arginine dihydrolyase : negative reaction, yellow colour or no colour change
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Motility : (+)ve
Arginine dihydrolyase : negative reaction, yellow colour or no colour change
Organism
Salmonella serotype Typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Motility : (+)ve
Arginine dihydrolyase : positive reaction, purple clour
Organism
Klebsiella pneumoniaeATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Motility : (--)ve
Arginine dihydrolyase : negative reaction, yellow colou or no colour change
Key
Motility :
(+)ve : positive, growth away from stabline
(--)ve : negative, growth alongwith stabline
Applications
Used for the detection of arginine dihydrolase producing microorganisms.
Composition
Ingredients
gms/lt.
Peptic digest of animal tissue
1.00
Sodium chloride
5.00
Dipotassium hydrogen phosphate
0.30
L-Arginine
10.00
Bromocresol Purple
0.016
Agar
3.00
Directions
1. Add 19.31 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 10 psi pressure at 115°C for 15 minutes.
5. Cool the tubes in upright position.
