Appearance (Colour)
White to off white
Appearance (Form)
Powder
Activity
min. 45 units/mg protein
Protein content
~91%
Unit Definition
(After activation to Chymotrypsin) One unit is that amount of enzyme causing a decrease in absorbance at 237 nm of 0.0075 per minute at pH 7.0 at 25°C using N-Acetyl-L-Tyrosine Ethyl Ester (ATEE) as a substrate.
Enzyme solution for activity determination
Prepared in 1 mM HCl.
Application
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond. Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges and requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form Α, β, γ , δ, and π chymotrypsin (depending upon the conditions of activation).
