Appearance (Colour)
Off white
Appearance (Form)
Free flowing, homogeneous powder
Solubility
19.01 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Very light amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours (with added sodium selenite) and sub culture on MacConkey Agar after incubation at 35-37°C for 18-24 hours.
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : Increase in numbers
Colony colour on MacConkey Agar : colourless
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : Increase in numbers
Colony colour on MacConkey Agar : colourless
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Good
Recovery Rate : none to poor
Colony colour on MacConkey Agar : pink w/bile precipitate
Inhibited
Applications
Used for enrichment of Salmonella species.
Composition
Part A
Ingredients
gm/lt.
Peptone
5.00
Dulcitol
4.00
Disodium phosphate
10.00
Part B
Sodium selenite
4.00
Directions
1. Add 19.00 gm powder to distilled/purified water.
2. Add 4.00 gm Sodium selenite powder to Part A.
3. Dispense into test tubes.
4. Sterilize in boiling water bath or free flowing steam for 10 minutes.
Caution :
*Sodium Selenite is very toxic. Avoid contact with skin and eyes, incase of contact wash immediately with water and seek medical advice.
