Appearance (Colour)
Reddish brown
Appearance (Form)
Free, flowing homogeneous powder
Solubility (Colour) 2% aq. solution
Light to medium amber, clear
pH (25°C)
6.0 - 6.5 (2.0 aq.%)
Stability (After autoclaving)
Light to medium amber, clear
Loss on drying
<= 5.0 %
Nitrogen (N) content
9.5 - 11.0 %
NaCl
< 5.0%
Ash
< 13.6%
Total Aerobic microbial count
(By Plate Count Method) : <10,000 cfu/gm
Total Yeast and Mould count
(By Plate Count Method) : <100 cfu/gm
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours with the following organisms
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Applications
Nutritive characteristic are good in Liver extract so used for cultivation of anaerobic bacteria such as Brucellae and Clostridia, also used for cultivation of pathogenic bacteria.
